Description of Individual Course Units
Course Unit CodeCourse Unit TitleType of Course UnitYear of StudySemesterNumber of ECTS Credits
9303135022006Applications of Rekombinant DNA TechnologyCompulsory1212
Level of Course Unit
Second Cycle
Objectives of the Course
“Gene Engineering Applications” is a continuation of first semester “Gene Engineering” Lectures with a particular emphasis on experimental applications of the former one. The students are aimed to gain the capability of DNA manipulation of the topics covered in “Gene Engineering” course.
Name of Lecturer(s)
Prof Dr. Zeki Topçu
Learning Outcomes
1Comprehension of basic recombinant biotechnology techniques
2Gaining of molecular genetic fundamentals and interpetation.
3Improvement of personal vision on biotechnology applications, and improving research capabilities on pharmacutical biotechnology.
4
5
Mode of Delivery
Face to Face
Prerequisites and co-requisities
None
Recommended Optional Programme Components
None
Course Contents
Gel analyses of DNA, Restrictional analyses, Translation, Gene cloning, DNA amplification, DNA library and library screening, Drug-DNA interactions.
Weekly Detailed Course Contents
WeekTheoreticalPracticeLaboratory
1Restriction enzymesPractical
2Restrictional length polymorphismPractical
3Genetic codeLecture
4TranslationLecture
5RibosomesLecture
6Agarose gel electrophoresisPractical
7First examExam
8Polyacrylamide gel electrophoresisPractical
9DNA ligase applications, cohesive and blunt end ligation.Lecture
10Polymerase Chain ReactionPractical
11Parameters affecting the fidelity of Polymerase Chain reaction.Practical
12Preparation and screening of genomic and cDNA libraries.Lecture
13Effects of anti-tumor drugs on DNA topoisomerase-catalyzed reactionsPractical
14Formation of single- and double-stranded breaks on DNA by topoisomerase enzymesPractical
15Gene therapyLecture
16Final examExam
Recommended or Required Reading
Sambrook et al, 1993. Molecular Cloning. Cold Spring Harbor Laboratory Press. New York
Planned Learning Activities and Teaching Methods
Activities are given in detail in the section of "Assessment Methods and Criteria" and "Workload Calculation"
Assessment Methods and Criteria
Term (or Year) Learning ActivitiesQuantityWeight
SUM0
End Of Term (or Year) Learning ActivitiesQuantityWeight
SUM0
SUM0
Language of Instruction
Turkish
Work Placement(s)
None
Workload Calculation
ActivitiesNumberTime (hours)Total Work Load (hours)
Midterm Examination144
Final Examination188
Attending Lectures14570
Self Study8756
Individual Study for Homework Problems6742
TOTAL WORKLOAD (hours)180
Contribution of Learning Outcomes to Programme Outcomes
PO
1
PO
2
PO
3
PO
4
PO
5
PO
6
PO
7
PO
8
PO
9
PO
10
PO
11
PO
12
LO14 3  4 5 53 
LO2544 3 333  5
LO34543   4 4 3
LO4            
LO5            
* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High
 
Ege University, Bornova - İzmir / TURKEY • Phone: +90 232 311 10 10 • e-mail: intrec@mail.ege.edu.tr