Description of Individual Course Units
Course Unit CodeCourse Unit TitleType of Course UnitYear of StudySemesterNumber of ECTS Credits
9102015171998Tissue Culture Applications in Horticulture IElective117
Level of Course Unit
Second Cycle
Objectives of the Course
The aim of this course is to let the students to gain knowledge and skills on basic concept and principles of tissue culture and learn how to realize tissue culture of horticultural crops.
Name of Lecturer(s)
Learning Outcomes
1
2
3
4
5
6
7Being able to perform tissue culture and apply it in practice
8Being able to observe and evaluate plant development
9Being able to select the right medium for tissue culture
10Being able to recognize the importance of tissue culture technique
11Being able to found the laboratory and understanding the lab. needs
12Understanding the components fo the tissue culture medium
13Understanding and solving the problems confronted with in tissue culture
Mode of Delivery
Face to Face
Prerequisites and co-requisities
None
Recommended Optional Programme Components
None
Course Contents
Description, history and the importance of tissue culture, techniques of tissue culture to manage the labaratory, contents of media, preperation of nutrient medium, sterilization of plant material, inoculation, subculture, factors affecting growth and development
Weekly Detailed Course Contents
WeekTheoreticalPracticeLaboratory
1Introduction and history of tissue cultureIntroduction and Literature cited
2Tissue culture methods and applicationsLaboratory planning
3Laboratory desingProcedure, essential equipment and suppliers
4Laboratory equipmentSuggestions for media prepagation
5Compotents of nutrient mediumPreparation and sterilization methods
6Preparation of nutrient mediumControlling contaminants
7Sterilization of nutrient mediumSterilization by filtration
8Midterm ExamLaboratory work
9Methods and chemicals used in plant starilizationİnitiating tissue cultures
10Explant isolation, induction and subcultureLaboratory work,
11The influence of plant material and physical factors on growthThe cultural environment
12Physological disordersProblems of establishment ,initiating and maintaining
13Presentation of term-work projectsLaboratory work
14Subculturing mechanization and automationSubculturing
15Transfer from soil to nutrient mediumAcclimatization
16Final ExamExam for the practical work
Recommended or Required Reading
Plant propagation by tissue culture Part 1 - The Technology (1993) Plant propagation by tissue culture Part 2 - In Practice (1993-1996) Edwin F. George, Exegetics Ltd. Micropropagation Technology and Application (1991) Deberg, P.C. and R.H. Zimmerman, Kluwer Academic Publishers. In Vitro Culture of Higher Plants (1989) Pierik, R.L.M., Martinus Nijhoff Publishers. Experiments in Tissue Culture (1985) Dodds, J. And Roberts, W.L., Cambridge Univ. Press.
Planned Learning Activities and Teaching Methods
Activities are given in detail in the section of "Assessment Methods and Criteria" and "Workload Calculation"
Assessment Methods and Criteria
Term (or Year) Learning ActivitiesQuantityWeight
SUM0
End Of Term (or Year) Learning ActivitiesQuantityWeight
SUM0
SUM0
Language of Instruction
Turkish
Work Placement(s)
None
Workload Calculation
ActivitiesNumberTime (hours)Total Work Load (hours)
Midterm Examination000
Final Examination000
Attending Lectures000
Practice000
Project Preparation000
Project Presentation000
Self Study14456
Individual Study for Mid term Examination000
Individual Study for Final Examination000
Laboratory Midterm Examination000
Laboratory Examination122
TOTAL WORKLOAD (hours)58
Contribution of Learning Outcomes to Programme Outcomes
PO
1
PO
2
PO
3
PO
4
PO
5
PO
6
PO
7
PO
8
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LO2        
LO3        
LO4        
LO5        
LO6        
LO7        
LO8        
LO9        
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LO11        
LO12        
LO13        
* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High
 
Ege University, Bornova - İzmir / TURKEY • Phone: +90 232 311 10 10 • e-mail: intrec@mail.ege.edu.tr