Description of Individual Course Units
Course Unit CodeCourse Unit TitleType of Course UnitYear of StudySemesterNumber of ECTS Credits
9102015432010Plant Gene Expression and Analysis TechniquesElective118
Level of Course Unit
Second Cycle
Objectives of the Course
The aim of the course is to teach the mechanism of gene expression in prokaryot ve eucaryots, the function of proteins involved in basic, active and chromatin transcription, mRNA processing, degradation, localization, gene silencing and the regulation of gene expression and analysis techniques.
Name of Lecturer(s)
Assist.Prof. Dr. Birsen ÇAKIR
Learning Outcomes
1Ability to analyse gene expression in plants
2Ability to use the basic principles of gene expression
3Ability to make a relation between gene expression and plant physiology
4Ability to develop and perform molecular procedures to solve problems in plant production
Mode of Delivery
Face to Face
Prerequisites and co-requisities
None
Recommended Optional Programme Components
None
Course Contents
Transcription, cromatin and gene silencing, mRNA processing and splicing, regulation of Gene expression, gene expression analysis techniques.
Weekly Detailed Course Contents
WeekTheoreticalPracticeLaboratory
1Overview of Gene Expression And transcriptionRNA Polymerases seminar
2Transcription Mechanisms in Prokaryotes Transcriptional Termination in Prokaryotes Regulation of lac Operon Transcription seminar
3Gene's Regulatory Elements Promoter The lac Operon Promoter The IL-2 Promoter Enhancer Silencer Response Elements Agarose gel and electrophoresis techniques
4Transcription Mechanisms in EukaryotesAgarose gel and electrophoresis techniques
5Motif Structures of Transcription FactorsRNA isolation
6Histone Acetylation and DNA Methylation PCR technique
7Chromatin Structure and gene silencingPCR technique
8Midexam
9RNA Processing RNA Splicing transport and localization Seminar
10Regulation of gene expression Transcriptional and posttranscriptional regulation Seminar
11Translational and posttranslational regulationSeminar
12Gene expression analysis techniquesRT-PCR
13Northern and Dot blot analysisNorthern and Dot blot techniques
14RT-PCR and Real Time PCR seminar
15SeminarSeminar
16Final exam
Recommended or Required Reading
1. Regulation of gene expression: molecular mechanisms, Gary H. Perdew, John P. Vanden Heuvel, Jeffrey ... - 2006 2. RNA and the Regulation of Gene Expression: A Hidden Layer of Complexity, Caister Academic Pres Edited by: Kevin V. Morris The Scripps Research Institute, La Jolla, USA 2008, ISBN: 978-1-904455-25-7 3. Practical Applications of Plant Molecular Biology (Paperback), R. J. Henry, Publisher: Chapman & Hall (January 1, 1997) , ISBN-10: 0412732203 4. Real-Time PCR: Current Technology and Applications, Julie Logan, Kirstin Edwards and Nick Saunders : 2009 ISBN: 978-1-904455-39-4
Planned Learning Activities and Teaching Methods
Activities are given in detail in the section of "Assessment Methods and Criteria" and "Workload Calculation"
Assessment Methods and Criteria
Term (or Year) Learning ActivitiesQuantityWeight
SUM0
End Of Term (or Year) Learning ActivitiesQuantityWeight
SUM0
SUM0
Language of Instruction
Turkish
Work Placement(s)
None
Workload Calculation
ActivitiesNumberTime (hours)Total Work Load (hours)
Midterm Examination122
Final Examination122
Attending Lectures14228
Practice7214
Laboratory7214
Seminar177
Self Study148112
Individual Study for Mid term Examination12020
Individual Study for Final Examination13030
Homework21020
TOTAL WORKLOAD (hours)249
Contribution of Learning Outcomes to Programme Outcomes
PO
1
PO
2
PO
3
PO
4
PO
5
PO
6
PO
7
PO
8
LO1        
LO2        
LO3        
LO4        
* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High
 
Ege University, Bornova - İzmir / TURKEY • Phone: +90 232 311 10 10 • e-mail: intrec@mail.ege.edu.tr