Description of Individual Course Units
Course Unit CodeCourse Unit TitleType of Course UnitYear of StudySemesterNumber of ECTS Credits
402023422002MICROBIAL PHYSIOLOGYCompulsory365
Level of Course Unit
First Cycle
Objectives of the Course
Prokaryotes can be found almost everywhere including aerobic to anaerobic, light and dark, low to high pressure, low to high salt concentration and extremes of acidity and alkalinity. As a result of this, prokaryotic microorganisms exhibited incredible diversity regarding their metabolism and physiology. Some physiologies, e.g. lithotrophy, nitrification and nitrogen fixation are only found in certain groups of prokaryotes. The aim of this course is also to provide a general overview of metabolic diversity of different groups of prokaryotes.
Name of Lecturer(s)
Prof. Dr. İhsan YAŞA
Learning Outcomes
11. To obtain basic concepts used in the theory and practices of microbial physiology and establish necessary associations between them.
22. To comprehend their knowledge about the importance of microbial metabolic diversity in applied sciences.
33. To adapt knowledge of microbial metabolic diversity to the problems of industry, agriculture, health and environment
44. To learn the microbial responses to harmful conditions.
55. To interpret and evaluate the importance of protecting and the protection and survival of microbial biodiversity of our country.
66. Making students follow current developments in this field of research by reading/discussing new research papers.
77. Being able to share information about microbial physiology by oral and written language.
88. To understand the relations between experimental logic, analytical thinking, quantitative analysis and problem solving.
Mode of Delivery
Face to Face
Prerequisites and co-requisities
Recommended Optional Programme Components
none
Course Contents
Glucose oxidation to pyruvate (EMP, PP and ED pathways),energy transduction in prokaryotes, PMF in acidophiles and alkaliphiles, Anaerobic respiration(denitrification, metal reduction, sulphate reduction, methanogenesis, homoacetogenesis), Chemolithotrophy (nitrification, anammox, the oxidation of hydrogen and sulphur compounds), anoxygenic photosynthesis, nitrogen and sulphate assimilation;living strategies at high temperature, salt concentration in archael strains.
Weekly Detailed Course Contents
WeekTheoreticalPracticeLaboratory
11 Introduction to bacterial physiology and metabolism: Structure-functional relationship, importance of unique metabolic process in prokaryotes Asimilatif and dissimilatif metabolisms
22 Membrane transport (nutrient uptake) and protein excretion: Ionophores, ABC pathways, general secretory systems. Types of microbial nutrition
33 Types of Glycolysis in prokaryotes: EMP,PP and ED pathways; Tricarboxylic acid (TCA) cycle O/F and cytocrome oxsidase tests; anaerobik cultivation techniques
44 Electron transport and oxidative phosphorylation: Energy transduction in prokaryotes, ΔG0’from the free energy of formation, free energy of an oxidation/reduction reaction. Effects of environmental factors on microbial growth.
55 Biosynthesis and microbial growth: Sulfate assimilation, amino acid, nucleotide and lipid biosynthesis; synthesis of saccharides and their derivatives, cell division and growth yield Homo –heterolactic fermentations
66 Heterotrophic metabolism on substrates other than glucose: Hydrolysis of polymers, Organic acid and amino acid utilization, Oxidation of aliphatic hydrocarbons and aromatic compounds Preparations of buffer
77 PresentationMethods of protein determination (Lowry)
88 MidtermMidterm
99 Heterotrophic metabolism on substrates other than glucose: Utilization of methane and methanol, Incomplete oxidations Methods of protein determination (Bradford )
1010 Anaerobic fermentation: Molecular oxygen and anaerobes ethanol lactate fermentation, butyrate and acetone–butanol–ethanol, mixed acid, butanediol, propionate fermentation, Fermentation of amino acids and dicarboxylic acids. Determination of Alfa-Amylase(Protease) activity
1111 Anaerobic respiration: Denitrifikasyon, metal reduction, sulfidogenesis, methanogenesis, homoacetogenesis, syntrophic associations, nitrate reduction. Enzyme purification
1212 Chemolithotrophy: Nitrification, the oxidation of sulfur compounds, ferrous iron and hydrogen; fixation pathways in chemolithotrophs.Effect of pH on enzyme activity and stability
1313 Photosynthesis in prokaryotes: Photosynthetic microorganisms, photosynthetic pigments and apparatus, Light reactions, carbon metabolism in phototrophs Effect of temperature on enzyme activity and stability
1414 Metabolic regulation :regulation and biochemistry of N2 fixation, Global regulation: responses to environmental stress Microbial growth and kinetics
1515 PresentationExam preperation
1616 Final Exam Final Exam
17
Recommended or Required Reading
Lecture Book: Kim, B. Hand Gadd, G.M. “Bacterial Physiology and Metabolism” Cambridge University Press, The Edinburgh Building, Cambridge CB2 8RU, UK, 2008. Further readings: Yaşa,İ., Çadırcı,B.H.,”Mikrobiyal Fizyoloji Uygulama Kitabı” E.Ü. Yayınları, Fen Fakültesi Yayın No:204,198 sayfa, ISNB:978-975-483-757-5, E.Ü.Basımevi Bornova -İzmir.2007 Dworkin,M. , Falkow, S., Rosenberg, E.,Schleifer, K.H., Stackebrandt ,E. “The Prokaryotes Volume 2: Ecophysiology and Biochemistry” Springer Science-Business Media, LLC. ,2006 Larry L., Barton. “Structural and Functional Relationships in Prokaryotes”. Springer Science-Business Media, Inc., 2005.
Planned Learning Activities and Teaching Methods
Activities are given in detail in the section of "Assessment Methods and Criteria" and "Workload Calculation"
Assessment Methods and Criteria
Term (or Year) Learning ActivitiesQuantityWeight
SUM0
End Of Term (or Year) Learning ActivitiesQuantityWeight
SUM0
SUM0
Language of Instruction
Turkish
Work Placement(s)
none
Workload Calculation
ActivitiesNumberTime (hours)Total Work Load (hours)
Midterm Examination11212
Final Examination11212
Attending Lectures14228
Laboratory14228
Report Preparation236
Report Presentation224
Project Preparation339
Project Presentation212
Individual Study for Mid term Examination428
Reading12224
Homework4312
TOTAL WORKLOAD (hours)145
Contribution of Learning Outcomes to Programme Outcomes
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* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High
 
Ege University, Bornova - İzmir / TURKEY • Phone: +90 232 311 10 10 • e-mail: intrec@mail.ege.edu.tr