Description of Individual Course Units
Course Unit CodeCourse Unit TitleType of Course UnitYear of StudySemesterNumber of ECTS Credits
10420221E11424MICROBIAL GENETICSCompulsory487
Level of Course Unit
First Cycle
Objectives of the Course
The aim of this course is to learn the basic concepts of microbial genetics, regulation of the gene expression in prokaryotic and eukaryotic microorganisms, the mutations and repair mechanisms in microorganisms, the molecular mechanisms and functions of homologous and site specific recombination in microorganisms, Bacteriophage genetics and basic molecular mechanisms, molecular mechanisms of gene transfer mechanisms such as transformation, conjugation and transduction, microbial genomics and basic genetic engineering tools.
Name of Lecturer(s)
Professor Ataç UZEL
Learning Outcomes
1To understand the basic concepts and molecular mechanisms in microbial genetics.
2To understand the gene transfer mechanisms between microorganisms.
3To make connection between microbial genetic themes with different disciplines such as molecular biology, biochemistry and statistics.
4To understand the connection between microbial genetics and genetic engineering.
5To be able to apply the microbial genetic themes to current life.
6To be able to make connection between the experiments and knowledge in microbial genetic themes.
7To be able to research the microbial genetic databases and can transfer the gained knowledge by orally and literally.
8To be able to make connection between experimental logic, analytic thinking and problem solving.
Mode of Delivery
Face to Face
Prerequisites and co-requisities
None
Recommended Optional Programme Components
None
Course Contents
Regulation of gene expression of prokaryotes; DNA binding proteins, negative and pozitive control, binding of activator proteins, signal transduction, global regulatory mechanisms, antisense RNA, riboswitchs, attenuation. Molecular mechanisms and functions of RNA splicing and alternative splicing in microorganisms. Mutations and repair mechanisms in microorganisms. Molecular mechanism and functions of homologous recombination. Bacteriophage genetics; the topology and topography of phage genome, complementation, gene regulation, details of infection process, regulation of T4 gene expression, introns in T4 and a new process of splicing. Transformation. Plasmids. Conjugation. Transduction. Transposons and other mobile genetic elements, site specific recombination. Microbial genomics. Genetic engineering.
Weekly Detailed Course Contents
WeekTheoreticalPracticeLaboratory
1Regulation of gene expression in prokaryotes; DNA binding proteins; Repression and induction; Positive control; Binding of activator proteins; Signal transduction; Global regulation mechanisms; Antisens RNA, Riboswitchs, Atenuation.Laboratory rules and preparation
2Molecular mechanisms of RNA splicing and its functionsGenomic DNA isoaltion from prokaryotes
3Molecular mechanisms of alternative splicing and its functionsPlasmid DNA isolation from prokaryotic microorganisms
4Mutations and repair mechanisms in microorganisms. Genomic DNA isoaltion from eukaryotic microroganisims
5Molecular mechanisms and functions of homologous recombination in microorganisms. Analysis of DNA, purity and quantity check.
6Bacteriophage genetics, topology and topography of the phage genome, complementation, general genomic structure and gene arrangement, details of the infection process, regulation of T4 gene expression, T4 introns and a novel splicing mechanism. PCR application
7TransformationElectrophoresis of DNA and PCR products.
8MidtermFluctuation assay and Antibiotic resistant mutants.
9Plasmids Phenotypic modifications
10Conjugation IReplica Plate, Marker control.
11Conjugation II Transformation
12Transduction Conjugation
13Transposons and other mobile genetic elements, site specific recombination. Marker control for Ames test.
14Microbial Genomic Ames Test
15Genetic engineering Laboratory exam
16Final exam Laboratory exam II
Recommended or Required Reading
• Snyder, L. and Chapness, L. “Molecular Genetics of Bacteria”. 2nd Ed. ASM pres, Washington D.C. USA. 2003. • Birge, E. A. “Bacterial and Bacteriophage Genetics” 5th Ed. Springer. USA. 2006 • Streips, U.N. and Yasbin, R.E. “Modern Microbial Genetics” 2nd Ed. Wiley-Liss Inc. New York. USA. 2002 • Madigan, M.T ve Martinko, J.M. “Biology of Microorganisms” 12th Ed.
Planned Learning Activities and Teaching Methods
Activities are given in detail in the section of "Assessment Methods and Criteria" and "Workload Calculation"
Assessment Methods and Criteria
Term (or Year) Learning ActivitiesQuantityWeight
SUM0
End Of Term (or Year) Learning ActivitiesQuantityWeight
SUM0
SUM0
Language of Instruction
English
Work Placement(s)
None
Workload Calculation
ActivitiesNumberTime (hours)Total Work Load (hours)
Attending Lectures14228
Practice3412
Laboratory14228
Report Preparation224
Self Study4416
Individual Study for Homework Problems5210
Individual Study for Mid term Examination13030
Individual Study for Final Examination13636
Reading11444
Report224
TOTAL WORKLOAD (hours)212
Contribution of Learning Outcomes to Programme Outcomes
PO
1
PO
2
PO
3
PO
4
PO
5
PO
6
PO
7
PO
8
PO
9
PO
10
PO
11
PO
12
PO
13
PO
14
LO15 5        45 
LO2 5  3   55    
LO335       55   
LO4  5    3 54   
LO54 5           
LO6   5 55       
LO7  5           
LO8              
* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High
 
Ege University, Bornova - İzmir / TURKEY • Phone: +90 232 311 10 10 • e-mail: intrec@mail.ege.edu.tr