Description of Individual Course Units
Course Unit CodeCourse Unit TitleType of Course UnitYear of StudySemesterNumber of ECTS Credits
9101036052003Immobilized BiocatalystsElective118
Level of Course Unit
Third Cycle
Language of Instruction
Turkish
Objectives of the Course
The aim of is to provide students with a depth of knowledge of biocatalyst immobilization and their applications in different fields of biological sciences and industry. To present such information at a level suitable for students derived from a range of undergraduate programs and to a depth appropriate for students who will progress to advanced enzymology work.
Name of Lecturer(s)
Prof.Dr. Azmi Telefoncu
Learning Outcomes
1Be skilled in using, developing immobilization methods for applications in different fields of biological sciences and industry.
2Be able to read scientific literature searching and interpret the knowledge on biocatalyst immobilization
3Be able to follow up recent results and improve specific techniques on biocatalyst immobilization.
4Gain an ability to design a project, carry out experiments and evaluate results through becoming specialized in biocatalyst immobilization.
5Be skilled in integrating knowledge of Biocatalyst immobilization and gain experience.
6Be able to collaborate effectively as a team worker in disciplinary and interdisciplinary fields.
Mode of Delivery
Face to Face
Prerequisites and co-requisities
None
Recommended Optional Programme Components
None
Course Contents
Introduction, immobilization methods for enzymes, organelles, cells, and coenzymes, characterization of immobilized biocatalysts, applications of immobilized biocatalysts in industry, analysis, medicine and basic research.
Weekly Detailed Course Contents
WeekTheoreticalPracticeLaboratory
1Introduction to Biocatalyst Immobilization; Comparison of native and immobilized enzymesLiterature survey
2Classification of enzyme immobilization methods, carriers for enzyme immobilizationLiterature survey
3Enzyme Immobilization Methods; carrier binding method ; Carrier activation ; acylation, alkylation and arylation, carbamylation, aldehydes and etc.Covalent enzyme immobilization; activation of carrier
4Enzyme Immobilization Methods; Covalent binding method, Adsorption methodCovalent enzyme immobilization; Analysis of samples and immobilization yield
5Enzyme Immobilization Methods; Cross linking method, Copolymerization MethodCovalent enzyme immobilization; Characterization, Report preparation and Presentation
6Enzyme Immobilization Methods; Entrapment in polymer matrix, microencapsulation, Enzyme immobilization by enterapment in polymer matrix;
7Comparison of immobilization methods Enzyme immobilization by enterapment in polymer matrix;Analysis of samples and immobilization yield
8Midterm ExamEnzyme immobilization by enterapment in polymer matrix; Characterization, Report preparation and Presentation
9Characterization of immobilized enzymes; Determination of Immobilization yield, physical and chemical characterizationComparison of enzyme immobilization techniques by means of immobilization yield and effectiveness, Report preparation and Presentation
10Characterization of immobilized enzymes; effect of immobilization on enzyme activity, stability tests, temperature, pH, kinetic parametersStability tests of immobilized enzyme preparates; Storage, pHand thermal
11Selection of immobilization method and carrierStability tests of immobilized enzyme preparates; Storage(cont.), operational, repeatability. Report preparation and Presentation
12Applications of immobilized biocatalysts; therapy, artificial organs, analysis, preparation of biosensors, various industries and etc.Cell immobilization by enterapment in polymer matrix; Characterization, Report preparation and Presentation
13Class Discussion Discussion and tutorial
14Project presentationDiscussion and tutorial
15Project presentationDiscussion and tutorial
16Final Exam
Recommended or Required Reading
K. Andreas(Ed.), “Immobilized Catalysts, Solid Phases, Immobilization and Applications Series: Topics in Current Chemistry”, Vol. 242, (2004) A. Telefoncu(Ed.), “Enzimoloji”, Ege Üniversitesi Baskı Atölyesi, (1997) G. F. Bickersteff, “Immobilization of Enzymes and Cells”, Vol. 1, Humana Press, (1997) A. Tanaka, J. Tosa, T. Kobayashi, “Industrial Application of Immobilized Biocatalysts”, Marcel Decker, (1992)
Planned Learning Activities and Teaching Methods
Activities are given in detail in the section of "Assessment Methods and Criteria" and "Workload Calculation"
Assessment Methods and Criteria
Term (or Year) Learning ActivitiesQuantityWeight
Midterm Examination1100
SUM100
End Of Term (or Year) Learning ActivitiesQuantityWeight
Final Sınavı1100
SUM100
Term (or Year) Learning Activities40
End Of Term (or Year) Learning Activities60
SUM100
Work Placement(s)
None
Workload Calculation
ActivitiesNumberTime (hours)Total Work Load (hours)
Midterm Examination122
Final Examination122
Attending Lectures14456
Laboratory14342
Tutorial326
Discussion326
Team/Group Work5210
Report Preparation51050
Report Presentation515
Project Preparation12020
Project Presentation122
Criticising Paper10110
Individual Study for Mid term Examination11515
Individual Study for Final Examination12525
TOTAL WORKLOAD (hours)251
Contribution of Learning Outcomes to Programme Outcomes
PO
1
PO
2
PO
3
PO
4
PO
5
PO
6
PO
7
PO
8
PO
9
PO
10
PO
11
PO
12
LO1      5  4  
LO2 3 5        
LO3 35     55  
LO4     5   5 5
LO55   5       
LO6       5  5 
* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High
 
Ege University, Bornova - İzmir / TURKEY • Phone: +90 232 311 10 10 • e-mail: intrec@mail.ege.edu.tr