Description of Individual Course Units
Course Unit CodeCourse Unit TitleType of Course UnitYear of StudySemesterNumber of ECTS Credits
9101036181998Recombinant DNA EnzymologyElective128
Level of Course Unit
Third Cycle
Language of Instruction
Turkish
Objectives of the Course
This course aims to provide a comprehensive knowledge in molecular enzymology in recombinant DNA technology
Name of Lecturer(s)
Prof. Dr. Zeki TOPÇU
Learning Outcomes
1Students will have advance level of knowledge about enzymes of restriction, ligation and DNA modification.
2Students will have advance level of knowledge on enzymes of DNA cloning
3Students will develop new scientific methods for their experiments in biochemistry.
Mode of Delivery
Face to Face
Prerequisites and co-requisities
None
Recommended Optional Programme Components
None
Course Contents
Nukleases, restriction endonucleases, Taq DNA polymerase, DNA ligase, proteolitic enzymes, terminal deoksyribonucleotidyl transferase, DNAase and RNAase, alkalene phosphatase and kinase
Weekly Detailed Course Contents
WeekTheoreticalPracticeLaboratory
1TerminologyLecture
2Methods of recombinant DNA ILecture
3Methods of recombinant DNA IILecture
4Nucleases: deoksiribonuklease I,Bal31 nuclease, ming-bean nuclease Lecture
5Restriction endonucleaseLecture
6Restriction endonucleaseLecture
7Taq Dna Polymerase and PCRLecture- Discussion
8First Exam
9DNA and RNA ligaseLecture
10Proteolitic enzymesLecture- Discussion
11Homopolimer tailingLecture
12DNAase,RNAaseLecture
13Using of DNAase for footprint Lecture- Discussion
14Alcalen phosphataseLecture
15Using of KinaseLecture
16Final Exam
Recommended or Required Reading
Sambrook et al, (1993). Molecular Cloning. Cold Spring Harbor Laboratory Press. New York
Planned Learning Activities and Teaching Methods
Activities are given in detail in the sections of "Assessment Methods and Criteria" and "Workload Calculation".
Assessment Methods and Criteria
Term (or Year) Learning ActivitiesQuantityWeight
Midterm Examination1100
SUM100
End Of Term (or Year) Learning ActivitiesQuantityWeight
Final Sınavı1100
SUM100
Term (or Year) Learning Activities40
End Of Term (or Year) Learning Activities60
SUM100
Work Placement(s)
None
Workload Calculation
ActivitiesNumberTime (hours)Total Work Load (hours)
Midterm Examination122
Final Examination122
Attending Lectures14342
Question-Answer14228
Criticising Paper14228
Self Study14798
Individual Study for Mid term Examination12020
Individual Study for Final Examination12020
TOTAL WORKLOAD (hours)240
Contribution of Learning Outcomes to Programme Outcomes
PO
1
PO
2
PO
3
PO
4
PO
5
PO
6
PO
7
PO
8
PO
9
PO
10
PO
11
PO
12
LO14 3 54 3  45
LO24  5  4  5 4
LO343   5  5  5
* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High
 
Ege University, Bornova - İzmir / TURKEY • Phone: +90 232 311 10 10 • e-mail: intrec@mail.ege.edu.tr