Description of Individual Course Units
Course Unit CodeCourse Unit TitleType of Course UnitYear of StudySemesterNumber of ECTS Credits
9101036191998Gene EngineeringElective118
Level of Course Unit
Third Cycle
Language of Instruction
Turkish
Objectives of the Course
The aims of the course are: -to introduce the diverse strategies and methodologies employed in gene clonning, -to provide a deeper understanding of the fundamental principles of related techniques of molecular genetics and applications of these in various areas of biotechnology and medical science, -the promotion of human resources development by expanding and diversifying the pool of highly qualified trained students.
Name of Lecturer(s)
Prof. Dr. Zeki Topçu
Learning Outcomes
1Improvement of personal vision on gene engineering and biotechnology applications, and improving research capabilities.
2Comprehension of basic gene engineering processes
3Gaining of molecular genetic fundamentals and interpetation.
Mode of Delivery
Face to Face
Prerequisites and co-requisities
None
Recommended Optional Programme Components
None
Course Contents
Structure of genetic material, genetic information, gene clonning metodology, viral pasmid and cosmid vestors, enzymes that are used in gene clonning, biochemical analysis of clonning products, genomic and cDNA libraries, Analysis of DNA and RNA molecules by radioactive and nonradiactive methods, polimerase chain reaction and DNA nucleotide sequencing, invitro mutagenesis, binding of clonned genes to expressed vectors, application fields of genetic engineering.
Weekly Detailed Course Contents
WeekTheoreticalPracticeLaboratory
1Structure of genetic material.Lecture
2Genetic informationLecture
3Gene clonning metodology Lecture
4Viral pasmid and cosmid vectors.Lecture
5Enzymes that are used in gene clonningLecture
6Genomiclibrary Lecture
7cDNA librariesLecture
8First exam
9Analysis of DNA and RNA molecules by radioactive and nonradiactive methods. Lecture
10Polimerase Chain Reaction.Lecture
11DNA nucleotide sequencing Lecture
12invitro mutagenesis.Lecture
13Binding of clonned genes to expressed vectors. Lecture
14Application fields of genetic engineeringDiscussion
15Application fields of genetic engineeringDiscussion
16Final exam
Recommended or Required Reading
Glick & Pasternak “Molecular Biotechnology” ASM Press Washington, D.C. 1994 Lodish et al Molecular Cell Biology Freeman Press New York 2000
Planned Learning Activities and Teaching Methods
Activities are given in detail in the sections of "Assessment Methods and Criteria" and "Workload Calculation".
Assessment Methods and Criteria
Term (or Year) Learning ActivitiesQuantityWeight
Midterm Examination1100
SUM100
End Of Term (or Year) Learning ActivitiesQuantityWeight
Final Sınavı1100
SUM100
Term (or Year) Learning Activities40
End Of Term (or Year) Learning Activities60
SUM100
Work Placement(s)
None
Workload Calculation
ActivitiesNumberTime (hours)Total Work Load (hours)
Midterm Examination122
Final Examination122
Attending Lectures14456
Discussion14342
Criticising Paper14456
Individual Study for Mid term Examination13535
Individual Study for Final Examination14040
TOTAL WORKLOAD (hours)233
Contribution of Learning Outcomes to Programme Outcomes
PO
1
PO
2
PO
3
PO
4
PO
5
PO
6
PO
7
PO
8
PO
9
PO
10
PO
11
PO
12
LO145 55    5 5
LO2455   5  5 5
LO353 5 5 44 55
* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High
 
Ege University, Bornova - İzmir / TURKEY • Phone: +90 232 311 10 10 • e-mail: intrec@mail.ege.edu.tr