Description of Individual Course Units
Course Unit CodeCourse Unit TitleType of Course UnitYear of StudySemesterNumber of ECTS Credits
9101036402010Recombinant Protein Expression SystemsElective128
Level of Course Unit
Third Cycle
Language of Instruction
Turkish
Objectives of the Course
Giving information about the prokaryotic and eukaryotic expression systems used for heterologous protein expression constitutes the aim of the course. The target of the course is making the students able to compare the advantages/disadvantages of different expression systems and learn the broad application area of recombinant protein production.
Name of Lecturer(s)
Doç. Dr. Serap Evran
Learning Outcomes
1Be able to understand the general properties of recombinant protein expression systems and their differences.
2Be able to use the knowledge about recombinant protein expression in other research fields.
3Be able to have the knowledge level to follow up recent developments in recombinant protein expression.
4Be able to understand the issues regarding the choice of expression system
5Be able to read, understand and interpret the scientific papers on recombinant protein expression.
Mode of Delivery
Face to Face
Prerequisites and co-requisities
None
Recommended Optional Programme Components
None
Course Contents
Criteria to the selection of appropriate expression system, expression vectors, strategies for recombinant protein production and purification, fusion proteins, prokaryotic and eukaryotic hosts in use.
Weekly Detailed Course Contents
WeekTheoreticalPracticeLaboratory
1E. coli expression systemIntroduction
2Yeast expression systemsLiterature search-Literature reporting
3Yeast expression systemsLiterature search-Literature reporting
4Baculovirus expression system. Literature search-Literature reporting
5Protein expression in mammalian cells. Literature search-Literature reporting
6Recombinant protein production in plant cellsLiterature search-Literature reporting
7Cell-free protein expressionLiterature search-Literature reporting
8Midterm examLiterature search-Literature reporting
9Affinity tags for purification of recombinant proteins Literature search-Literature reporting
10Oral presentationOral presentation
11Oral presentationOral presentation
12Oral presentationOral presentation
13Oral presentationOral presentation
14Oral presentationOral presentation
15Oral presentation
16Final exam
Recommended or Required Reading
Doyle, S.A., 2009, “High Throughput Protein Expression and Purification: Methods and Protocols, Humana Press, London, U.K. Gellissen, G., 2004, “Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems”. Wiley-VCH Verlag GmbH, Weinheim. Baneyx, F., 2004, “Protein Expression Technologies: Current Status and Future Trends”. Horizon Bioscience, Norfolk, U.K. Cell-Free Protein Expression, James R. Swartz (Ed.), 2003, Springer. Protein Expression Technologies: Current Status and Future Trends, Francois Baneyx, 2004, Garland Science Protein Analysis and Purification: Benchtop Techniques, Ian M. Rosenberg, 2005, Birkhäuser
Planned Learning Activities and Teaching Methods
Assessment Methods and Criteria
Term (or Year) Learning ActivitiesQuantityWeight
Midterm Examination1100
SUM100
End Of Term (or Year) Learning ActivitiesQuantityWeight
Final Sınavı1100
SUM100
Term (or Year) Learning Activities40
End Of Term (or Year) Learning Activities60
SUM100
Work Placement(s)
None
Workload Calculation
ActivitiesNumberTime (hours)Total Work Load (hours)
Midterm Examination122
Final Examination122
Attending Lectures14228
Question-Answer14114
Report Preparation13452
Report Presentation111
Self Study14228
Reading14798
TOTAL WORKLOAD (hours)225
Contribution of Learning Outcomes to Programme Outcomes
PO
1
PO
2
PO
3
PO
4
PO
5
PO
6
PO
7
PO
8
PO
9
PO
10
PO
11
PO
12
LO1  3  3      
LO25      5 4 4
LO3    335     
LO4  3  3      
LO5   53   5 4 
* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High
 
Ege University, Bornova - İzmir / TURKEY • Phone: +90 232 311 10 10 • e-mail: intrec@mail.ege.edu.tr