Description of Individual Course Units
Course Unit CodeCourse Unit TitleType of Course UnitYear of StudySemesterNumber of ECTS Credits
9101036422011Protein Folding Elective128
Level of Course Unit
Third Cycle
Language of Instruction
Turkish
Objectives of the Course
The aim of this course is giving information about mechanisms of in vitro and in vivo protein folding and spectroscopic techniques used in folding and stability studies.
Name of Lecturer(s)
Doç. Dr. Serap EVRAN
Learning Outcomes
1Be able to read and understand scientific papers about protein folding.
2Be able to understand the impact of protein misfolding
3Be able to understand the importance of relationship between protein sturucture and function.
4Be able to describe the basic concepts of kinetic and thermodynamic mechanisms for protein folding
5Be able to follow up the recent developments in protein folding and stability.
Mode of Delivery
Face to Face
Prerequisites and co-requisities
None
Recommended Optional Programme Components
None
Course Contents
Factors affecting protein folding, protein folding in vivo, helper proteins for folding, folding of membrane proteins, protein misfolding diseases, spectroscopic techniques to study protein folding and stability, denaturation of proteins by urea and guanidine hydrochloride, thermal denaturation.
Weekly Detailed Course Contents
WeekTheoreticalPracticeLaboratory
1Secondary and tertiary structure of proteins, importance of protein folding and stability studies.
2Factors affecting protein folding and stability
3Protein folding problem and Levinthal’s paradox
4Mechanisms of protein folding
5Spectroscopic Techniques to Study Protein Folding
6Denaturation of proteins by urea and guanidine hydrochloride.
7Protein folding in vivo. Chaperon proteins
8Midterm exam
9Folding of membrane proteins
10Folding of Multi-domain and Oligomeric Proteins
11Protein misfolding
12Oral presentation
13Oral presentation
14Oral presentation
15Oral presentation
16Final Exam
Recommended or Required Reading
Buchner, J., Kiefhaber, T., 2005, “Protein Folding Handbook”. WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Nölting, B., 2005, “Protein Folding Kinetics: Biophysical Methods”. Springer-Verlag Berlin Heidelberg. Whitford, D., 2005, “Proteins Structure and Function”. WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Protein Folding: An Introduction (SpringerBriefs in Molecular Science), Cláudio M. Gomes, Patrícia F.N. Faísca, 2019, Springer,
Planned Learning Activities and Teaching Methods
Assessment Methods and Criteria
Term (or Year) Learning ActivitiesQuantityWeight
Midterm Examination1100
SUM100
End Of Term (or Year) Learning ActivitiesQuantityWeight
Final Sınavı1100
SUM100
Term (or Year) Learning Activities40
End Of Term (or Year) Learning Activities60
SUM100
Work Placement(s)
None
Workload Calculation
ActivitiesNumberTime (hours)Total Work Load (hours)
Midterm Examination122
Final Examination122
Attending Lectures14342
Question-Answer14114
Report Preparation12525
Report Presentation122
Self Study14456
Individual Study for Mid term Examination12525
Individual Study for Final Examination13030
Reading14228
TOTAL WORKLOAD (hours)226
Contribution of Learning Outcomes to Programme Outcomes
PO
1
PO
2
PO
3
PO
4
PO
5
PO
6
PO
7
PO
8
PO
9
PO
10
PO
11
PO
12
LO1   5  5   4 
LO2  4     5  5
LO34   4    5  
LO4   4 4 4   4
LO545 5  5 5 4 
* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High
 
Ege University, Bornova - İzmir / TURKEY • Phone: +90 232 311 10 10 • e-mail: intrec@mail.ege.edu.tr