Description of Individual Course Units
Course Unit CodeCourse Unit TitleType of Course UnitYear of StudySemesterNumber of ECTS Credits
9101036242012Advanced Biochemical Techniques Elective128
Level of Course Unit
Third Cycle
Language of Instruction
Turkish
Objectives of the Course
The advances in the biological sciences have largely depended on parallel advances in the technology of measuring and observing devices. Knowledge of these devices, new instrumentation methods and the basic principles behind analytical and industrial procedures will assist in general understanding of the equipment selection and an appreciation of good quality assurance and research procedures. The aims are to provide students with depth knowledge of a number of advanced techniques in biochemistry and to develop advanced practical skills for the handling and analysis of biomolecules.
Name of Lecturer(s)
Prof.Dr. Figen Zihnioglu
Learning Outcomes
1Gain important research experience and a better understanding of modern biochemical techniques
2Be able to explain the basic principles, operation and applications behind modern biochemical instrumentation techniques
3Be able to understand newer advances in biochemistry instrumentation technology, techniques of analytical biochemistry
4Gain an insight into the quantitative and qualitative determination and purification of biomolecules.
5Be able to understand the new diagnostic tools in modern biochemical laboratories
6Gain an ability to write scientific reports accurately and concisely.
7Be skilled in following up and selection of new techniques
Mode of Delivery
Face to Face
Prerequisites and co-requisities
None
Recommended Optional Programme Components
None
Course Contents
Basics of several recent biochemical techniques will be given prior to expertise in related techniques. Use of HPLC and electrophoretic methods for the aim of quantification and structure analysis, advanced mass spectrometric techniques and applications in Biochemistry, blotting, immuno-staining techniques, Multi Dimensional Techniques, Stable Isotope labeling Techniques, Imaging Techniques, 3D structure analysis and prediction of biomolecules, and related spectral techniques and X-Ray Christallography, immunobiochemical techniques, micro arrays and etc
Weekly Detailed Course Contents
WeekTheoreticalPracticeLaboratory
1Basic principles and applications of Hypnated techniques such as LC-MS/MS, CE-MS/MS
2Basic principles and applications of Advanced Mass Spectrometric Techniques such as MALDI-TOF, ESI-TOF, SELDI-TOF
3Basic principles and applications of Multi dimensional techniques such as, 2D-PAGE, 2D-DIGE, 2D-LC
4Basic principles and applications of Stable Isotope Labeling techniques and Mass Spectrometry such as ICAT, SILAC
5Basic principles and applications of Immunostaining techniques; ELISA, RIA
6Basic principles and applications of Spectral Techniques; Fluorescence, NMR, CD, FRET
7Class Discussion and seminar on reading assignment
8Midterm Exam
9Basic principles and applications of X-Ray Crystallography
10Basic principles and applications of Imaging Techniques such as Cyro EM, Immunofluorescence,
11Basic principles and applications of microarrays; DNA arrays, protein arrays, glycoarrays
12Class Discussion and seminar on reading assignment
13Evaluation, comprehension and comparison of techniques
14Project presentation
15Project presentation
16Final Exam
Recommended or Required Reading
A.Pingoud, C.Unbanke, J.Hogget, A.Jeltsch,“Biochemical Methods”,Wiley-VCH, (2001) J. P. Chapman, “Mass Spectrometry of Proteins and Peptides”, Humana Press, Totowa NJ, (2000) Textbooks and related articles will be chosen according to the developments Please consult your lecturer
Planned Learning Activities and Teaching Methods
Activities are given in detail in the sections of "Assessment Methods and Criteria" and "Workload Calculation".
Assessment Methods and Criteria
Term (or Year) Learning ActivitiesQuantityWeight
Midterm Examination1100
SUM100
End Of Term (or Year) Learning ActivitiesQuantityWeight
Final Sınavı1100
SUM100
Term (or Year) Learning Activities40
End Of Term (or Year) Learning Activities60
SUM100
Work Placement(s)
None
Workload Calculation
ActivitiesNumberTime (hours)Total Work Load (hours)
Midterm Examination133
Final Examination133
Attending Lectures14342
Tutorial339
Discussion339
Project Preparation12020
Project Presentation122
Seminar236
Self Study14342
Individual Study for Mid term Examination11515
Individual Study for Final Examination12525
Reading14456
TOTAL WORKLOAD (hours)232
Contribution of Learning Outcomes to Programme Outcomes
PO
1
PO
2
PO
3
PO
4
PO
5
PO
6
PO
7
PO
8
PO
9
PO
10
PO
11
PO
12
LO15 445 5    5
LO2454 4       
LO3     55 33  
LO4 4   4      
LO5  5         
LO642        4 
LO7   4    5  4
* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High
 
Ege University, Bornova - İzmir / TURKEY • Phone: +90 232 311 10 10 • e-mail: intrec@mail.ege.edu.tr