Description of Individual Course Units
Course Unit CodeCourse Unit TitleType of Course UnitYear of StudySemesterNumber of ECTS Credits
9103106212014Cell Culture Techniques in PlantsElective118
Level of Course Unit
Third Cycle
Objectives of the Course
The aim of the course is to make students aware of cell culture techniques in plants and to illustrate their application fields.
Name of Lecturer(s)
Prof.Dr. Aynur GÜREL
Learning Outcomes
14. Comprehending knowledge about advantages and disadvantages of plant cell culture techniques.
26- Being aware of new progresses and researches in plant cell culture techniques.
35- Gaining ability to evaluate the differences between plant cell culture techniques.
41- Comprehending importance of plant cell culture.
52- Comprehending general information on plant cell culture.
63. Gaining information of plant cell culture techniques.
Mode of Delivery
Face to Face
Prerequisites and co-requisities
None
Recommended Optional Programme Components
None
Course Contents
Introduction to plant cell culture techniques. Callus cultures. Suspension cultures. Isolation, culture and regenaration of protoplasts. Protoplast fusion. Somatic embryogenesis. Somatic organogenesis. Some exogenous (environmental) and endogenous (genetical) factors in cell culture systems. Cryopreservation. Cell division, growth and differentiation. Secondary metabolite production: Molecular and cellular approaches. Large scale cultures. Selection of plant cells for desirable characteristics. New progresses and researches related with plant cell culture techniques.
Weekly Detailed Course Contents
WeekTheoreticalPracticeLaboratory
1Introduction to plant cell culture techniques.
2Callus cultures.
3Suspension cultures.
4Isolation, culture and regenaration of protoplasts.
5Protoplast fusion.
6Somatic embryogenesis.
7Somatic organogenesis.
8Midterm Exam
9Some exogenous (environmental) and endogenous (genetical) factors in cell culture systems.
10Cryopreservation.
11Cell division, growth and differentiation.
12Secondary metabolite production: Molecular and cellular approaches.
13Large scale cultures.
14Selection of plant cells for desirable characteristics.
15New progresses and researches related with plant cell culture techniques
16Final Exam
Recommended or Required Reading
Neumann, K.H., A. , Kumar, J.Imani, 2009. Plant Cell and Tissue Culture- A Tool in Biotechnology: Basics and Application. Springer-Verlag, Berlin Heidelberg. Loyola-Vargas, V.M., F., Vázquez-Flota, 2006. Plant Cell Culture Protocols. Humana Press Inc., Totowa.
Planned Learning Activities and Teaching Methods
Activities are given in detail in the section of "Assessment Methods and Criteria" and "Workload Calculation"
Assessment Methods and Criteria
Term (or Year) Learning ActivitiesQuantityWeight
SUM0
End Of Term (or Year) Learning ActivitiesQuantityWeight
SUM0
SUM0
Language of Instruction
Turkish
Work Placement(s)
None
Workload Calculation
ActivitiesNumberTime (hours)Total Work Load (hours)
Midterm Examination122
Final Examination122
Attending Lectures14228
Report Preparation14747
Report Presentation14141
Individual Study for Mid term Examination16060
Individual Study for Final Examination16060
TOTAL WORKLOAD (hours)240
Contribution of Learning Outcomes to Programme Outcomes
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PO
13
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* Contribution Level : 1 Very low 2 Low 3 Medium 4 High 5 Very High
 
Ege University, Bornova - İzmir / TURKEY • Phone: +90 232 311 10 10 • e-mail: intrec@mail.ege.edu.tr